Table 2. Crystallization.

Method	Sitting-drop vapour diffusion
Plate type	MRC Maxi 48-well
Temperature (K)	293
Protein concentration (mg ml−1)	10
Buffer composition of protein solution	20 mM Tris–HCl pH 7.4, 0.1 M NaCl, 2 mM DTT
Composition of reservoir solution	39.3%(v/v) ethylene glycol, 0.1 M MES pH 5.6, 0.2 M zinc acetate
Volume and ratio of drop	2 µl, 1:1
Volume of reservoir (µl)	210