Table 3. Adnectin selection conditions.

Condition	Target concentration (nM)	Incubation time (min)	Selection buffer†
A	100	60	Reducing selection buffer
B	100	30	Reducing selection buffer
C	100	30	PBSA
D	10	30	PBSA
E	1	30	PBSA

^†Reducing selection buffer is composed of 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 137 mM NaCl, 2.7 mM KCl, 2 mM DTT, 0.125% Tween-20, 1 mg ml⁻¹ BSA, 0.1 mg ml⁻¹ sheared salmon sperm DNA. PBSA is composed of 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 137 mM NaCl, 2.7 mM KCl, 0.1%(w/v) BSA.