Figure S4.

Nanoscale displacement of fluorescent-tagged components within individual plus-end-attached kinetochore assemblies during periodic flow-induced reorientation. Positions for the indicated GFP-labeled components were tracked with subpixel accuracy while the direction of fluid flow was oscillated, causing the kinetochore and its captured microtubule to flip back and forth, reorienting by 180° with each reversal of the flow. Displacements from the tether point were estimated by averaging during the intervals when the microtubule orientation was steady. Black symbols represent mean ± SD from N = 60 tracked positions during each interval. Positions recorded during the reorientation of the microtubule were omitted from averaging and are indicated here by gray shading.