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. 2024 Nov 1;224(1):e202405176. doi: 10.1083/jcb.202405176

Table 1.

Reagents and resources

Reagent or resource Source Identifier
Antibodies
His Tag biotinylated antibody R&D Systems BAM050
Biological samples
Purified tubulin from bovine brains Schenk Packing Co. NA
Yeast whole-cell lysate Listed strains NA
Chemicals, peptides, and recombinant proteins
Tubulin protein (fluorescent HiLyte 647): Porcine brain Cytoskeleton, Inc. TL670M
Tubulin protein (biotin): Porcine brain Cytoskeleton, Inc, T333P
Vectabond reagent, tissue section adhesion Vector Laboratories SP-1800-7
mPEG-SVA MW 5,000 Laysan Bio, Inc. mPEG-succinimidyl valerate
Biotinylated mPEG-SVA MW 5,000 Laysan Bio, Inc. Biotin-PEG-SVA
Avidin DN Vector Laboratories A-3100-1
Biotin-BSA Vector Laboratories B-2007-10
Paclitaxel Millipore Sigma T7191-5MG
Guanosine 5′-triphosphate sodium salt hydrate Milipore Sigma G8877-1G
Streptavidin-coated polystyrene beads Spherotech SVP-05-10
Guanosine-5′-[(α,β)-methyleno]triphosphate, sodium salt (GMPcPP) Jena Bioscience JBS-NU-405S
Experimental models: Organisms/strains
S. cerevisiae: MATa ura3-1::pCSE4-CSE4-XbaI(GFP):URA3 leu2,3-112 his3-11 trp1-1 ade2-1 LYS2+ can1-100 bar1 cse4Δ::KanMX Biggins lab SBY19926
S. cerevisiae: MATa pDsn1-Dsn1-2D-3FLAG:URA3 Ndc10-GFP:kanMX6 Biggins lab SBY21618
S. cerevisiae: MATa pDsn1-Dsn1-2D-3FLAG:URA3 Ndc80-GFP:kanMX6 Biggins lab SBY21620
S. cerevisiae: MATa pDsn1-Dsn1-2D-3FLAG:URA3 Ctf19-GFP:kanMX6 Biggins lab SBY21621
S. cerevisiae: MATx pDsn1-Dsn1-2D-3FLAG:URA3 Dam1-3GFP:HIS Biggins lab SBY20634
Oligonucleotides
Forward primer for generating 208-bp CEN3 or CEN3mut: /5BiosG/5′-GGTGGTT​CTGG​TGGTT​CTGG​TGAA​TTC-CCATT​CAATG​AAAT​ATATA​TTTC​TTACT​AT​TTC-3′ This paper 50_JDL
Reverse primer for generating 208-bp CEN3 or CEN3mut: /5Atto565N/5′-GCT​ATT​CAT​TGA​AAAA​ATAG​TACA​AAT​AAG-3′ This paper 52_JDL
5′ primer to tag Ndc80: 5′-ACG​AAA​TTT​GGA​GTT​TGA​AAC​TGA​ACA​TAA​CGT​AAC​AAA​TCG​GAT​CCC​CGG​GTT​AAT​TAA-3′ This paper 30_JDL
3′ primer to tag Ndc80: 5′-CTG​TAG​ATT​GCT​CGG​GTA​TTA​TAT​ATC​ATT​TAT​TTT​ATT​AGA​ATT​CGA​GCT​CGT​TTA​AAC-3′ This paper 31_JDL
5′ primer to tag Ctf19: 5′-GAT​CTG​CAA​CGT​TTG​CCT​ATT​CCC​GGA​CAT​GTA​CGC​CAG​GCG​GAT​CCC​CGG​GTT​AAT​TAA-3′ This paper 10_JDL
3′ primer to tag Ctf19: 5′-TAA​GCA​AGC​CGT​CCA​GTT​GGC​AAT​GGC​AAA​TGG​AAC​ATC​AGA​ATT​CGA​GCT​CGT​TTA​AAC-3′ This paper 11_JDL
5′ primer to tag Ndc10: 5′-TCA​AAA​TTC​ATT​TGA​TGG​TCT​GTT​AGT​ATA​TCT​ATC​TAA​CCG​GAT​CCC​CGG​GTT​AAT​TAA-3′ This paper 6_JDL
3′ primer to tag Ndc10: 5′-TAT​CCC​TAT​ACG​AAA​CAG​TTT​AAA​CTT​CGA​AGC​TCC​CTC​AGA​ATT​CGA​GCT​CGT​TTA​AAC-3′ This paper 7_JDL
Recombinant DNA
pSB963: WT CEN3, 8LacO, TRP1 Lang et al. (2018) NA
pSB972: Mutant CEN3 (CCG->AGC CEN mutant), 8 LacO, TRP1 Lang et al. (2018) NA
Software and algorithms
Single molecule colocalization analysis (written in LabView) This paper https://github.com/casbury69/smTIRF-spot-selection-colocalization-and-brightness-vs-time
Displacement analysis (written in Igor Pro) This paper https://github.com/casbury69/flip-flop-IGOR-analysis-routines
ImageJ (Fiji) Schindelin et al. (2012) https://imagej.net/software/fiji/
MOSAICsuite Sbalzarini and Koumoutsakos (2005) https://imagej.net/plugins/mosaicsuite