Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1984 Mar 1;218(2):557–562. doi: 10.1042/bj2180557

Antizyme to ornithine decarboxylase is present in the liver of starved rats.

K Fujita, S Matsufuji, Y Murakami, S Hayashi
PMCID: PMC1153372  PMID: 6712631

Abstract

Antizyme to ornithine decarboxylase (ODC) and ODC-antizyme complex were both present in liver cytosols of starved rats. The antizyme was identified by its molecular weight, kinetic properties, formation of a complex with ODC, and reversal of its inhibition by antizyme inhibitor. The average amount of antizyme in liver cytosols of starved rats was 0.1 unit/mg of protein, roughly corresponding to basal hepatic ODC activity in rats fed ad libitum. The presence of ODC-antizyme complex was detected by using antizyme inhibitor. These results indicate that antizyme participates in the regulation of ODC activity in vivo under physiological conditions.

Full text

PDF
557

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Branca A. A., Herbst E. J. Inhibition of ornithine decarboxylase of HeLa cells by diamines and polyamines. Effect on cell proliferation. Biochem J. 1980 Mar 15;186(3):925–931. doi: 10.1042/bj1860925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brosnan M. E., Farrell R., Wilansky H., Williamson D. H. Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary gland of lactating rats. Biochem J. 1983 Apr 15;212(1):149–153. doi: 10.1042/bj2120149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark J. L., Fuller J. L. Regulation of ornithine decarboxylase in 3T3 cells by putrescine and spermidine: indirect evidence for translational control. Biochemistry. 1975 Oct 7;14(20):4403–4409. doi: 10.1021/bi00691a010. [DOI] [PubMed] [Google Scholar]
  4. Fong W. F., Heller J. S., Canellakis E. S. The appearance of an ornithine decarboxylase inhibitory protein upon the addition of putrescine to cell cultures. Biochim Biophys Acta. 1976 Apr 23;428(2):456–465. doi: 10.1016/0304-4165(76)90054-4. [DOI] [PubMed] [Google Scholar]
  5. Fujita K., Murakami Y., Hayashi S. A macromolecular inhibitor of the antizyme to ornithine decarboxylase. Biochem J. 1982 Jun 15;204(3):647–652. doi: 10.1042/bj2040647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hayashi S., Aramaki Y., Noguchi T. Diurnal change in ornithine decarboxylase activity of rat liver. Biochem Biophys Res Commun. 1972 Jan 31;46(2):795–800. doi: 10.1016/s0006-291x(72)80211-0. [DOI] [PubMed] [Google Scholar]
  7. Hayashi S., Fujita K. Antizyme and antizyme inhibitor of ornithine decarboxylase (rat liver). Methods Enzymol. 1983;94:185–193. doi: 10.1016/s0076-6879(83)94031-4. [DOI] [PubMed] [Google Scholar]
  8. Hayashi S., Kameji T. Ornithine decarboxylase (rat liver). Methods Enzymol. 1983;94:154–158. doi: 10.1016/s0076-6879(83)94024-7. [DOI] [PubMed] [Google Scholar]
  9. Heller J. S., Canellakis E. S. Cellular control of ornithine decarboxylase activity by its antizyme. J Cell Physiol. 1981 May;107(2):209–217. doi: 10.1002/jcp.1041070206. [DOI] [PubMed] [Google Scholar]
  10. Heller J. S., Fong W. F., Canellakis E. S. Induction of a protein inhibitor to ornithine decarboxylase by the end products of its reaction. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1858–1862. doi: 10.1073/pnas.73.6.1858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heller J. S., Kyriakidis D., Fong W. F., Canellakis E. S. Ornithine decarboxylase antizyme is a normal component of uninduced H-35 cells and rat liver. Eur J Biochem. 1977 Dec;81(3):545–550. doi: 10.1111/j.1432-1033.1977.tb11980.x. [DOI] [PubMed] [Google Scholar]
  12. Hietala O. A. Detection of ornithine decarboxylase antizyme in mouse brain. J Neurochem. 1983 Apr;40(4):1174–1177. doi: 10.1111/j.1471-4159.1983.tb08110.x. [DOI] [PubMed] [Google Scholar]
  13. Icekson I., Kaye A. M. In vitro inactivation of ornithine decarboxylase by a heat-labile factor from rat ventral prostate. FEBS Lett. 1976 Jan 1;61(1):54–58. doi: 10.1016/0014-5793(76)80170-6. [DOI] [PubMed] [Google Scholar]
  14. Jänne J., Hölttä E. Regulation of ornithine decarboxylase activity by putrescine and spermidine in rat liver. Biochem Biophys Res Commun. 1974 Nov 27;61(2):449–456. doi: 10.1016/0006-291x(74)90977-2. [DOI] [PubMed] [Google Scholar]
  15. Kameji T., Murakami Y., Hayashi S. Effect of diaminobutene and diaminopropane on diet-stimulated polyamine synthesis and cell proliferation in rat liver. J Biochem. 1979 Jul;86(1):191–197. [PubMed] [Google Scholar]
  16. Kay J. E., Lindsay V. J. Control of ornithine decarboxylase activity in stimulated human lymphocytes by putrescine and spermidine. Biochem J. 1973 Apr;132(4):791–796. doi: 10.1042/bj1320791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Maudsley D. V. Regulation of polyamine biosynthesis. Biochem Pharmacol. 1979;28(2):153–161. doi: 10.1016/0006-2952(79)90496-9. [DOI] [PubMed] [Google Scholar]
  19. McCann P. P., Tardif C., Hornsperger J. M., Böhlen P. Two distinct mechanisms for ornithine decarboxylase regulation by polyamines in rat hepatoma cells. J Cell Physiol. 1979 May;99(2):183–190. doi: 10.1002/jcp.1040990204. [DOI] [PubMed] [Google Scholar]
  20. McCann P. P., Tardif C., Mamont P. S. Regulation of ornithine decarboxylase by ODC-antizyme in HTC cells. Biochem Biophys Res Commun. 1977 Apr 25;75(4):948–954. doi: 10.1016/0006-291x(77)91474-7. [DOI] [PubMed] [Google Scholar]
  21. Morris D. R., Fillingame R. H. Regulation of amino acid decarboxylation. Annu Rev Biochem. 1974;43(0):303–325. doi: 10.1146/annurev.bi.43.070174.001511. [DOI] [PubMed] [Google Scholar]
  22. Noguchi T., Aramaki Y., Kameji T., Hayashi S. Correlation between circadian rhythms of polyamine synthesis and cell proliferation in rat liver. J Biochem. 1979 Apr;85(4):953–959. doi: 10.1093/oxfordjournals.jbchem.a132427. [DOI] [PubMed] [Google Scholar]
  23. Obenrader M. F., Prouty W. F. Detection of multiple forms of rat liver ornithine decarboxylase. J Biol Chem. 1977 May 10;252(9):2860–2865. [PubMed] [Google Scholar]
  24. Russell D. H., Snyder S. H. Amine synthesis in regenerating rat liver: extremely rapid turnover of ornithine decarboxylase. Mol Pharmacol. 1969 May;5(3):253–262. [PubMed] [Google Scholar]
  25. Tabor C. W., Tabor H. 1,4-Diaminobutane (putrescine), spermidine, and spermine. Annu Rev Biochem. 1976;45:285–306. doi: 10.1146/annurev.bi.45.070176.001441. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES