Fig 4. Annexin V/PI assay of the methanolic extract of O. ficus-indica (L.) Mill. flowers against different cancer cell lines.

(A) Dot plots of the flow cytometric analysis of the treated and untreated cells; the quadrant was set to indicate the live cells (lower left corner), early apoptosis (lower right corner), late apoptosis (upper right corner), and necrosis (upper left corner). (B) A graphic bar chart to show the percentages of total apoptosis (early and late apoptosis) of IC₅₀-treated and untreated cells. *** indicated the statistical significance of the results at a P-value <0.001 compared to control untreated cells.