Figure 4. Cryo-electron tomography imaging of glutamatergic synapses within brain tissue.

(A) Medium montage map image of lamella. (B _I-III) Enlarged images of lamella are indicated in A. (C _i-iii) Slices from SIRT-filtered tomograms were collected at squares indicated in B. The synaptic cleft in each tomogram is indicated with a black arrow. Mit: mitochondria and MT: microtubule side views. One gold fiducial is visible in C _iii. Scale bars are 100 nm. Each slice shown is about 10 nm thick. (D) Example image of myelin membranes (D₁), top-down view of microtubule (D₂), synaptic vesicles (D₃), and part of a mitochondria (D₄). Scale bars are 20 nm. Each slice shown is 10 nm thick.

Figure 4—figure supplement 1. Power spectra and contrast transfer function (CTF) fitting of tilt series.

IMOD calculation of Fast Fourier Transform, or FFT (left), and Ctfplotter fits (right) for three tilts from a single representative tilt series.