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. 2024 Aug 3;73(4):399–411. doi: 10.1538/expanim.23-0149

Fig. 2.

Fig. 2.

Hepatic functional parameters and senescence in female and male diethylnitrosamine (DEN)-treated mice. (a, e) The concentration of IL-6 (pg/ml) in liver lysates (a) and serum (e) from control and DEN-treated mice was evaluated by ELISA assay. (b) Concentration of IL-1β in liver lysates. Levels of IL-1β in serum were undetectable. (c) Concentration of HMGB1 in liver lysates. Data represent the mean ± SEM of IL-6, IL-1β, or HMGB1 of 5 mice per group. Statistical comparison between experimental groups and control: *P<0.05; **P<0.01; ***P<0.001. Statistical comparison among female and male groups at the same time: #P<0.05; ###P<0.001. (d) Percentage (%) of senescence in liver sections of female and male DEN-treated mice at 210-, and 300-days post–DEN administration. Data represent the mean ± SEM of senescence areas (%) per liver section of 5 mice per group. Statistical comparison among female and male groups at the same time: #P<0.05. (f) Alanine aminotransferase (ALT) and (g) aspartate aminotransferase (AST) levels (U/I) in the serum of control mice and mice treated with DEN. Data represent the mean ± SEM of ALT or AST levels of 5 mice per group. Statistical comparison among experimental groups: **P<0.01; ***P<0.001. Statistical comparison among female and male groups at the same time: ###P<0.001. All statistical analyses of this figure were performed using ANOVA followed by the Tukey test. F=female; M=male. The control group corresponds to day 210.