Table 2.
List of secondary antibodies used for Western blotting and immunohistochemistry
| Secondary Antibody | Company | Cat number | Dilution |
|---|---|---|---|
| Western Blotting | |||
| Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP | Invitrogen | 15217664 | 1:10,000 |
| Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody | Invitrogen | 15291378 | 1:10,000 |
| IRDye 800CW Donkey anti-Mouse |
Li-cor Biosciences |
925–32212 | 1:20,000 |
| Immunocytochemistry | |||
| Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 | Invitrogen | 10236882 | 1:1000 |
| Invitrogen Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 | Invitrogen | 10226882 | 1:1000 |