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. 2024 Jul 17;60(9):1058–1067. doi: 10.1007/s11626-024-00948-6

Table 2.

List of secondary antibodies used for Western blotting and immunohistochemistry

Secondary Antibody Company Cat number Dilution
Western Blotting
 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Invitrogen 15217664 1:10,000
 Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody Invitrogen 15291378 1:10,000
 IRDye 800CW Donkey anti-Mouse

Li-cor

Biosciences

925–32212 1:20,000
Immunocytochemistry
 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 Invitrogen 10236882 1:1000
 Invitrogen Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 Invitrogen 10226882 1:1000