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. 2024 Aug 19;43(21):4846–4869. doi: 10.1038/s44318-024-00196-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV1 — C57BL/6NCrSlc wild-type (WT) mice were fed with a normal chow (NC) or a high-fat diet (HFD) from 4 weeks of age and studied at 38–40 weeks of age. (A, B) Body weight (BW) (A) (n = 4, 6) or brown adipose tissue (BAT) weight (B) (n = 4, 5) of indicated mice. (C) Reference Expression Dataset (RefEx) showing transcript Pcolce in systemic organs. (D–J) Gene Expression Omnibus studies showing transcript Pcolce in eWAT and BAT (D) (n = 3, 3 in GSE8044). The transcript was also tested under a HFD fed condition in mice in BAT (E) (n = 3, 3 in GSE64718), eWAT (n = 4, 4), liver (n = 4, 4), skeletal muscle (SM) (F) (n = 3, 4 in GSE123394), bone (G) (n = 3, 4 in GSE194075), adrenal gland (H) (n = 4, 4 in GSE216327), skin (I) (n = 5, 5 in GSE96932) and in heart (J) (n = 4, 4 in GSE171710). (K–M) Tabula Muris Senis testing transcript Pcolce in systemic organs (K), or in cells in the heart (L) or BAT (M). (N–P) Results from quantitative PCR (qPCR) showing transcript Pcolce in primary cardiac fibroblasts and primary brown adipocytes (N) (n = 6, 6), primary hepatocytes and primary brown adipocytes (O) (n = 5, 5), and hepatocyte (AML12) or brown adipocyte cell lines (P) (n = 11, 12). (Q) Relative fibrotic area (area/view) in the liver of indicated mice related to Fig. 1J (n = 4, 5). Data were analyzed by an independent-samples T-test. Data information: Representative data of two or more independent series (EV1A, B, N, P, Q), one independent series (EV1O). *P < 0.05, **P < 0.01. NS = not significant. Values represent the mean ± SEM. All data are from different biological replicates. Source data are available online for this figure.