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. 2024 Aug 19;43(21):4846–4869. doi: 10.1038/s44318-024-00196-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV4 — (A) Results of in silico analysis (DBTSS [http://dbtss.hgc.jp] and JASPAR [http://jaspar.binf.ku.dk]) predicting c-Fos and c-Jun may bind to putative promotor region of Pcolce. (B) Western blot analysis for phospho-JNK1/2 (p-JNK1/2) and JNK1/2 in fully differentiated brown adipocytes administrated with or without palmitic acid (Palm, 100 μM). The right panel indicates the quantification of p-JNK1/2 (n = 6, 6) relative to GAPDH. (C–E) Western blot analysis for p-c-Fos, c-Fos, p-c-Jun, and c-Jun in fully differentiated brown adipocytes administrated with PBS, palmitic acid (Palm; 100 μM for 6 h) with or without an IRE1 inhibitor (STF-083010, 30 μM, pre-treated 1 h) (C) or a JNK inhibitor (SP600125, 2 μM, pre-treated 1 h) (D, E). The right panels indicate the quantification of p-c-Fos and p-c-Jun relative to GAPDH or Actin (C: n = 6, 6, 6 for p-c-Fos, n = 11, 12, 12 for p-c-Jun) (D: n = 4, 6, 6) (E: n = 5,6,6). (F–I) Results from quantitative PCR (qPCR) showing transcript Pcolce in the differentiated brown adipocytes administrated with Palm (E) (n = 6, 6), BRE (Brefeldin A) (G) (n = 4, 4), Tunicamycin (H) (n = 6, 8) or Thapsigargin (I) (n = 4, 4). Data in Fig. EV4C–E were analyzed by a two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. Other data were analyzed by an independent-samples T-test. Data information: Representative data of two or more independent series (EV4B–I). *P < 0.05, **P < 0.01. NS = not significant. Values represent the mean ± SEM. All data are from different biological replicates. Source data are available online for this figure.