Table 5.
Experimental outline of the environmental variables used for stress trials on adult green sea urchins Strongylocentrotus droebachiensis. Each environmental variable trial was conducted in triplicate.
| Experimental group | Sample time (h) | Total /tank | Total/3 tanks | ||||
|---|---|---|---|---|---|---|---|
| 0 | 3 | 6 | 9 | 12 | |||
| CONTROL | 30 | -- | -- | -- | -- | 10 | 30 |
| OUT OF WATER* | -- | 5 | 5 | 5 | 5 | 20 | 60 |
| WARM WATER (18⁰ C) * | -- | 5 | 5 | 5 | 5 | 20 | 60 |
| COLD WATER (8⁰C) * | -- | 5 | 5 | 5 | 5 | 20 | 60 |
| TOTAL SEA URCHINS | 210 | ||||||
*Triplicate tanks were initially stocked with 20 animals each for each experimental group. Five animals were sampled from a tank at each time interval.