Fig. 1.

Jolkinolide B attenuates hepatocellular lipotoxicity. (A) JB structure. (B) Hepatocytes were treated with JB for each concentration (1, 5, and 10 μM), and cell viability was measured. (C) Cytotoxicity assay in hepatocytes were incubated with PA for 24 h with different concentrations (0, 1, 5, and 10 μM) of JB. Low control refers to cells under normal conditions, while the High control represents 100% cell death. (D) Whole cell lysates from primary hepatocytes and Hep3B cells were extracted and analyzed by immunoblotting of Bax/Bcl-2 after PA (200 μM) and JB (10 μM). The data are expressed as means ± sem. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.