Fig. 4.

JB alleviates hepatic steatosis by preventing JAK/STAT3 activity. JB alleviates hepatic steatosis by preventing JAK/STAT3 activity. (A) Relative protein expression levels of JAK and STAT3 and β-Actin were analyzed as immunoblot data concentrations. Values are presented as the means ± SEM and results are expressed by at least three independent experiments. (B) The protein expression levels of pSTAT3 and PPARgamma for each concentration of Stattic (0, 1, 5, 10, and 20 μM) were confirmed by immunoblot. (C) After 24 h of treatment with either Stattic or JB in the PA-treated group in primary hepatocytes. (D) lipid accumulation contents were compared through BODIPY staining. The images were shown at 20× and 40× magnifications for each experimental condition (scale bar=100 μm). Relative mRNA expression levels were normalized to mouse GAPDH levels. The data are expressed as means ± sem. *p<0.05, **p<0.01, and ****p<0.0001.