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. 2024 Sep 25;43(21):5260–5287. doi: 10.1038/s44318-024-00214-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV4 — (A–C) Multiplexed detection of the effect of depletion of NELFB and NELFE on the expression levels of reporter genes randomly integrated throughout the genome in K562 cell pools. Barcoded reporters were driven by promoters from the ARHGEF9, BRINP1, and MED30 genes as indicated. Cell pools are from (Leemans et al, 2019). (A) Western blot of NELFE showing partial knockdown after siRNA-mediated depletion of NELFB or NELFE. (B) Changes in expression of the reporters for the three promoters throughout the genome correlate between NELFB and NELFE knockdowns. Reporters integrated in LADs are shown in green, reporters in iLADs are shown in gray. The gray and green lines represent a fitted linear model for iLAD and LAD integrations, respectively; Pearson correlation and P values are shown in the plots. (C) Changes in expression of each reporter (log₂ scale) after siRNA-mediated knock-down of NELFB (top panel) and NELFE (bottom panel), divided by location in either LADs or iLADs. Results are from three replicates for P_BRINP1 and two replicates for P_MED30 and P_ARHGEF9. P-values comparing the distributions in LADs and iLADs are according to Wilcoxon test. Source data are available online for this figure.