Figure 2.

Thrombogenicity of the DREAMS 3G prototype made from BIOmag material is significantly diminished. (A) Individual flow experiments were conducted with PRP obtained from the same buffy coat for all scaffolds. Laminar shear stress of 10dyn/cm² was applied for 2 h. Representative immunofluorescence of platelets attached to Magmaris-316 L and DREAMS 3G prototype (PT) scaffolds using an antibody against CD62P (green) and phalloidin (red, actin). (B) Quantification of platelet coverage (n = 3–6 images per scaffold from three independent experiments). Data represent mean ± s.e.m. p value, Two-tailed unpaired Student’s t-test. (C) Representative immunofluorescence of platelets attached to scaffold material in a reversible manner (category 1: rolling, ball-shaped and hemisphere-shaped) and in an irreversible manner (category 2: spreading platelet). (D) Irreversible platelet adhesion to the DREAMS 3G prototype scaffold is significantly diminished. Individual flow experiments were conducted with PRP obtained from the same buffy coat for all scaffolds. Laminar shear stress of 10dyn/cm² was applied for 2 h. Quantification of irreversibly attached platelets (category 2) (n = 4 images per scaffold from two independent experiments). Data represent mean ± s.e.m. p value, Two-tailed unpaired Student’s t-test. Scale bars: 50 μm (A), 5 μm (C).