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. 2024 Oct 22;8(12):bvae182. doi: 10.1210/jendso/bvae182

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Published by Oxford University Press on behalf of the Endocrine Society 2024.

This work is written by (a) US Government employee(s) and is in the public domain in the US. See the journal About page for additional terms.

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Figure 5. — Pyroptosis induction. A to F, Neutral thyrotropin receptor antibodies (N-TSHR-mAbs) induced B, caspase 8; C, gasdermin-D (GSDM-D); D, caspase-9; E, Bax; and F, cytochrome-C but not A, FADD, indicative of the onset of pyroptosis. *P less than .05. G, Immunochemistry revealed similar data. Red bar = 100 μm. H, Western blotting added further confirmation to these observations. MC (MCC950 = 5μM, NLRP3 inhibitor) and rotenone (Rot = 10μM) were used to suppress NLRP3 or to induce free radicals, respectively.