Table 1.
Antibodies used in the described methods
| Name | Host | Source | Catalog No. | ∼Dilution | |||
|---|---|---|---|---|---|---|---|
| ICC | ICW | WB | RRID | ||||
| IGF-1R-B-mAb, 1H7 IgG1 κ | Mouse | Santa Cruz Biotechnology Inc | SC461 | — | — | AB_671786 | |
| TSHR-St-mAb, M22 | Human | RSR Ltd | — | — | — | AB_2892140 | |
| TSHR-St-mAb, MS1 | Hamster | Davies Laboratory | — | — | — | AB_3552210 | |
| pMek1/2-Ab (S217/221) | Rabbit | Cell Signaling Technology | 9121 | — | 1:100 | 1:1000 | AB_331648 |
| pErk1/2-Ab (T202/Y204) | Rabbit | Cell Signaling Technology | 4370 | — | 1:100 | 1:1000 | AB_2315112 |
| pAkt-Ab (S473) | Rabbit | Cell Signaling Technology | 4060 | — | 1:100 | 1:1000 | AB_ 2315049 |
| pPI3K, p85 (Y458) | Rabbit | Cell Signaling Technology | 3821 | — | 1:100 | 1:1000 | AB_330320 |
| Mek1/2 (nonphospho) | Rabbit | Cell Signaling Technology | 8727 | — | 1:100 | 1:1000 | AB_ 10829473 |
| Erk1/2 (nonphopho) | Rabbit | Cell Signaling Technology | 4695 | — | 1:00 | 1:1000 | AB_ 390779 |
| Akt (nonphospho) | Rabbit | Thermo Fisher Scientific | 4685 | — | 1:100 | 1:1000 | AB_2225340 |
| PI3K, p85 (nonphospho) | Rabbit | Cell Signaling Technology | 4257 | — | 1:100 | 1:1000 | AB_ 659889 |
| Cytochrome-C | Rabbit | Cell Signaling Technology | 11940 | — | 1:100 | 1:1000 | AB_2637071 |
| Caspase-3 | Rabbit | Cell Signaling Technology | 14220 | — | 1:100 | 1:1000 | AB_2798429 |
| Caspase-9 | Rabbit | Cell Signaling Technology | 20750 | — | 1:100 | 1:1000 | AB_2798848 |
| Caspase-8 | Rabbit | Aviva Systems Biology Co | OAMA03439 | — | 1:100 | 1:1000 | AB_10873136 |
| FADD | Rabbit | Aviva Systems Biology Co | ARP30294 | — | 1:100 | 1:1000 | AB_938079 |
| pIGF-1R-Ab (Y1161) | Rabbit | Biomatik Co | 10895 | — | 1:100 | 1:1000 | AB_731544 |
| Beclin 1 | Rabbit | St Johns Laboratory | STJ97761 | 1:200 | 1:100 | 1:1000 | — |
| LC3A | Rabbit | St Johns Laboratory | STJ97755 | 1:200 | 1:100 | 1:1000 | — |
| LC3B, α MAP | Rabbit | St Johns Laboratory | STJ97398 | 1:200 | 1:100 | 1:1000 | — |
| ULK1 | Rabbit | Abclonal | A8529 | — | 1:100 | 1:1000 | AB_2772810 |
| P62 | Rabbit | Abclonal | A11483 | — | 1:100 | 1:1000 | AB_2861579 |
| Isotype control, IgG1, k-chain | Mouse | BD Bioscience | 562438 | — | — | AB_11207319 | |
| β-Actin-mAb | Mouse | Sigma Millipore | A1978 | — | — | 1:1000 | Ab_476692 |
Fluorescein isothiocyanate–conjugated goat antirabbit (1:200 dilution, Jackson Laboratory) for immunocytochemistry (ICC), horseradish peroxidase–conjugated antibody (Cell Signaling, 1:12 000) for Western blot (WB), and IRDye anti-rabbit (680/800, 1:800) for In-Cell Western (ICW, Li-Cor Biosciences).