Table 2.

Ibuprofen validation parameters in plasma (n = 5).

Intra-assay variability
Concentration (μg/mL)	Measured conc. (Mean ± SD)	Accuracy (%)	RSD (%)
0.05	0.05 ± 0.002	100	4.0
0.15	0.15 ± 0.01	100	9.9
7.5	8.2 ± 0.07	109	0.8
35	35 ± 1.3	100	3.8
75	76 ± 1.98	101	2.6
Inter-assay variability
0.05	0.05 ± 0.004	100	8.0
0.15	0.15 ± 0.01	100	6.2
7.5	7.5 ± 0.74	100	9.8
35	35 ± 1.6	100	4.6
75	76 ± 2.0	101	2.7
Recovery	Recovery ± SD	RSD (%)
0.05	98 ± 10.3	10.6
0.15	97 ± 10.1	10.0
7.5	95 ± 1.9	2.0
35	97 ± 5.3	5.4
75	96 ± 6.3	6.6

n: number of samples; SD: standard deviation; RSD: relative standard deviation.