Figure 1.

Enlarged cells surrounding the MMC were identified in mARF10_GFP lines

(A) ARF10_GFP expression at pre-meiosis is detected in a few cells surrounding the MMC (L1 and L2 layers).

(B and C) mARF10_GFP expression pattern (lines a and b), at pre-meiosis a strong GFP signal is detected in all ovule cells, including the MMC.

(D) DIC analysis in WT.

(E and F) DIC analysis in mARF10 lines a and b, respectively; white arrows indicate multiple enlarged cells.

(G) pKNU:nlsYFP expression in WT.

(H and I) pKNU:nlsYFP expression in mARF10 a and b, respectively; the YFP signal was detected only in one of the enlarged cells (white arrows), often displaced to one side by the presence of extra enlarged cells (red arrows).

(J–L) Meiotic marker ASY:RFP in WT and mARF10 lines. In the transgenic lines only one of the enlarged cells accumulated the meiotic precursor (white arrows), extra enlarged cell didn’t express it (red arrows).

(M–O) Renaissance staining in ovule primordia during meiosis; cell plates accumulating callose are detectable. In the WT context two central plates are identifiable; in the transgenic lines they are often displaced (white arrows), red arrows indicate the extra enlarged cells. Renaissance staining was used for the cell walls in all marker line backgrounds. GFP and YFP signals detects expression, RFP detects autofluorescence. Scale bars:10μm.