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Successful culture of an equine intestinal epithelial monolayer. Mature small intestinal enteroids (A) were grown in culture for 5–7 days. Enteroids were dissociated and split into fragments (B, *) and replated onto coated transwells. Confluent intestinal epithelial monolayers (C) were achieved in 5–7 days. Scale bar, as indicated. N = 4.
