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Scratch wound assays to monitor epithelial migration and restitution. After reaching 100% confluency, monolayers were scratched using a P200 pipette tip as shown in (A). Wounds were assessed every 6h for closure (B–D). Epithelial cells can be seen migrating to help close the defect over 12–24 h (C,D). Scale bar = 100 μm. n = 3.
