Table 4.

Identification of RNA transcript via RT-PCR in equine jejunum crypts, enteroids and jejunum monolayer cell culture.

Epithelial cell types	Crypts	Enteroids	Monolayer
Active stem cells
SOX-9	+	+	+
LGR5	+	+	+
Reserve stem cells
HOPX	+	+	+
LRIG	+	+	+
Epithelial cells—EPCAM	+	+	+
Absorptive enterocytes—SIM	+	+	+
Enteroendocrine cells—CGA	+	+	+
Paneth cells—LYZ	+	+	+
Proliferating cells—PCNA	+	+	+
Tight junction proteins
ZO-1	+	+	+
Occludin	+	+	+
Claudin-1	+	+	+
Quality control
β-actin	+	+	+
No template control	−	−	−

+ indicates the presence of corresponding amplicon as assessed via gel electrophoresis, − indicates the absence of a corresponding amplicon.