Table 2.
Target gene | Primer: | Sequences |
---|---|---|
miRNA-222 | Forward: | 5′-CCCTCAGTGGCTCAGTAG-3′ |
Reverse: | 5′-CCACCAGAGACCCAGTAG-3′ | |
miRNA-155 | Forward: | 5′-CGCGTTAATGCTAATCGTGATA-3′ |
Reverse: | 5′-AGTGCAGGGTCCGAGGTATT-3′ | |
Insulin receptor | Forward: | 5′-TTT TCG TCC CCA GGC CAT C-3′ |
Reverse: | 5′-GTC ACA TTC CCA ACA TCG CC-3′ | |
Insulin receptor substrate 1 | Forward: | 5′-TTTGAAGACCATAACCCACCAC-3′ |
Reverse: | 5′-ATTACACCAGTTCGTCCCTTTC-3′ | |
GADPH | Forward: | 5′-GTCTCCTCTGACTTCAACAGCG-3′ |
Reverse: | 5′-ACCACCCTGTTGCTGAGCCAA-3′ | |
RUN6B | Forward: | 5′-CTCGCTTCGGCAGCACA-3′ |
Reverse: | 5′AACGCTTCACGAATTTGCGT-3′ |
GADPH and RUN6B primers were used as endogenous control for mRNAs and miRNAs, respectively.