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. 2024 Nov 4;19:11772719241296619. doi: 10.1177/11772719241296619

Table 2.

Primers for miRNAs and mRNAs quantification using RT-qPCR.

Target gene Primer: Sequences
miRNA-222 Forward: 5′-CCCTCAGTGGCTCAGTAG-3′
Reverse: 5′-CCACCAGAGACCCAGTAG-3′
miRNA-155 Forward: 5′-CGCGTTAATGCTAATCGTGATA-3′
Reverse: 5′-AGTGCAGGGTCCGAGGTATT-3′
Insulin receptor Forward: 5′-TTT TCG TCC CCA GGC CAT C-3′
Reverse: 5′-GTC ACA TTC CCA ACA TCG CC-3′
Insulin receptor substrate 1 Forward: 5′-TTTGAAGACCATAACCCACCAC-3′
Reverse: 5′-ATTACACCAGTTCGTCCCTTTC-3′
GADPH Forward: 5′-GTCTCCTCTGACTTCAACAGCG-3′
Reverse: 5′-ACCACCCTGTTGCTGAGCCAA-3′
RUN6B Forward: 5′-CTCGCTTCGGCAGCACA-3′
Reverse: 5′AACGCTTCACGAATTTGCGT-3′

GADPH and RUN6B primers were used as endogenous control for mRNAs and miRNAs, respectively.