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Experimental design and workflow (created with BioRender.com). (A) Pooling of CSF in subsets of patients with ALL, a brain tumor or nontumor control. These disease-specific subgroup-pools were pooled 1:1:1 prior to each experiment and sequential ultracentrifugation. (B) workflow for tryptic digest methods. (C) Data acquisition modes. (D) workflow for data analysis. Created using BioRender.com.
