Figure 6.

Yoda1 facilitated renal injury and fibrosis by Bax mediated apoptosis and mitochondrial dysfunction. (A) The WB of Bax, Bcl2, Cytc, Caspase3 and Cleaved-caspase3 (n = 3 per group). (B) Bar plot represents the proteins quantification results of Bax, Bcl2, Cytc, Caspase3 and Cleaved-caspase3. (C) The mRNA expression level of Bax, Bcl2, Bax/Bcl2 and Caspase3 after Yoda1 treatment. (D) Flow cytometry scatter plot of AnnexinV-PI/7ADD staining in TCMK-1 cells after Yoda1 intervention (n = 3 per group). (E) the immunostaining of mitochondrial membrane potential after Yoda1 treatment (n = 3 per group, 3 random sights per sample), Scale bar 100 μm. (F) Detection of intracellular ATP content in TCMK-1 cells after Yoda1 intervention. (G) the intracellular calcium concentration was detected with fluorescence microscopy after Yoda1 intervention, Scale bar 100 μm. Statistical analyses were calculated using 2-tailed student’s t test for 2 groups. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001