Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Nov 5;57:78. doi: 10.1186/s40659-024-00559-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Representation of the integration of the Up-hph-pHMGS/crtE module in the X. dendrorhous genome. A Scheme illustrating the resulting product with the Up-hph-pHMGS/crtE module (digested with NotI and BglII enzymes), used to replace the native crtE gene promoter with the HMGS promoter through double homologous recombination. B PCR analysis of strains used in this work. A representation of the amplified fragment is provided beneath each gel, with primers indicated by numbered arrows according to Supplementary Table 1.2. Template DNA sources are as follows: CBS6938 (lane 1), CBS.pHMGS/crtE (lane 2), CBS.cyp61⁻ (lane 3), CBS.cyp61⁻.pHMGS/crtE (lane 4), CBS.SRE1N.FLAG (lane 5), and CBS.SRE1N.FLAG.pHMGS/crtE (lane 6). The GeneRuler 1 kb Plus DNA Ladder (M) was used as a molecular weight marker, and (−) represents a negative control without DNA in the PCR reaction