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. 2024 Nov 5;57:78. doi: 10.1186/s40659-024-00559-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Growth curves and color phenotype of strains. A Growth curves of strains CBS6938, CBS.cyp61⁻, CBS.SRE1N.FLAG and their respective mutants: CBS.pHMGS/crtE, CBS.cyp61⁻.pHMGS/crtE and CBS.SRE1N.FLAG. pHMGS/crtE. The strains were cultured in liquid YM medium at 22 °C with constant agitation, and growth was monitored at 600 nm. Growth rate (r) and generation time (t) were determined based on the method described by Zwietering et al. in 1990 [50], and values represent the average ± standard deviation of three simultaneous cultures for each strain. It should be noted that growth parameters were estimated only as an approximation to assess significant alterations in growth. No statistically significant differences in growth parameters were observed between mutant and their respective parental strain (p-value < 0.01). B Strains cultured on YM agar plates (1.5%) at 22 °C for 72 h numbered as follows: CBS6938 (1), CBS.pHMGS/crtE (2), CBS.cyp61⁻ (3), CBS.cyp61⁻.SRE1.pHMGS/crtE (4), CBS.SRE1N.FLAG (5), and CBS.SRE1N.FLAG.pHMGS/crtE (6)