Table 1.
Strains and plasmids used and built in this work
| Genotype or relevant features | Source or reference | |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Used for molecular cloning and plasmid maintenance | [29] |
| X. dendrorhous | ||
| CBS6938 | ATCC 96594, wild-type strain | ATTC |
| CBS.cyp61− | Zeor. Strain derived from strain CBS6938 in which the CYP61 gene locus was disrupted with a module that confers resistance to zeocin (Sh ble) | [24] |
| CBS.SRE1N.FLAG | Hygr. Strain derived from strain CBS6938 strain in which the SRE1 locus was replaced with a gene version that only expresses the active form of Sre1 (Sre1N) | [22] |
| CBS.pHMGS/crtE | Hygr. Strain derived from strain CBS6938, in which the crtE gene promoter was replaced with the HMGS gene promoter | This work |
| CBS.cyp61−.pHMGS/crtE | Zeor/Hygr. Strain derived from strain CBS.cyp61−, in which the crtE gene promoter was replaced with the HMGS gene promoter | This work |
| CBS.SRE1N.FLAG.pHMGS/crtE | Zeor/Hygr. Strain derived from strain CBS.SRE1N.FLAG, in which the crtE gene promoter was replaced with the HMGS gene promoter | This work |
| Plasmids | ||
| pBluescript SK- (pBS) | ColE1 ori; AmpR; cloning vector with blue-white selection | Stratagene |
| pBS-pHMGS/crtE | Plasmid pBlueScript SK- containing the module with the HMGS gene promoter (pHMGS) and the crtE gene segment (crtE*) at the EcoRV site | This work |
| pBS-Up-pHMGS/crtE | Plasmid pBlueScript SK-, containing the module with the crtE gene promoter upstream region (UP), the HMGS gene promoter (pHMGS) and the crtE gene segment (crtE*) at the EcoRV site | [24] |
| pBS-Up-hph-pHMGS/crtE | Plasmid pBlueScript SK-, containing the module comprising the crtE gene promoter upstream region (UP), the Hygromycin B resistance cassette (hph), the HMGS gene promoter (pHMGS) and the crtE gene segment (crtE*) at the EcoRV site | This work |
| pMN-hph | Plasmid pBlueScript SK-, containing the hygromycin B resistance cassette (1,8 kb) used for X. dendrorhous transformant selection at the EcoRV site | [51] |
CBS Centraalbureau voor Schimmelcultures, Utrecht Netherlands, ATCC American Type Culture Collection, Amps sensitive to the antibiotic ampicillin, Hygr resistant to the antibiotic hygromycin B, Zeor resistant to the antibiotic zeocin