Table-4.

Binomial test (proportion test) for comparison between positive and negative categories with respect to small ruminants and methods for different districts regarding prevalence.

Animal	Methods	Category	Muzaffargarh			Lodhran			Bahawalpur
			Prevalence	Proportion	p-value	Prevalence	Proportion	p-value	Prevalence	Proportion	p-value
Goat	Microscopic	Positive	3	0.19	0.021*	4	0.25	0.077NS	2	0.13	0.004**
		Negative	13	0.81		12	0.75		14	0.88
		Total	16	1.00		16	1.00		16	1.00
	PCR	Positive	2	0.13	0.004**	1	0.06	0.001**	1	0.06	0.001**
		Negative	14	0.88		15	0.94		15	0.94
		Total	16	1.00		16	1.00		16	1.00
Sheep	Microscopic	Positive	5	0.31	0.210NS	1	0.06	0.001**	3	0.19	0.021*
		Negative	11	0.69		15	0.94		13	0.81
		Total	16	1.00		16	1.00		16	1.00
	PCR	Positive	1	0.06	0.001**	0	1.00	0.000**	0	1.00	0.000**
		Negative	15	0.94		16	1.00		16	1.00
		Total	16	1.00		16	1.00		16	1.00

NS=Non-significant (p> 0.05),

^*Significant (p < 0.05),

^**Highly significant (p < 0.001), PCR=Polymerase chain reaction