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. 2024 Sep 15;17(9):2077–2087. doi: 10.14202/vetworld.2024.2077-2087

Table-3.

Amino acid composition of protein concentrate extracted from Indigofera leaves using distilled water and 0.1 N NaOH.

Amino acid (%) Indigofera leaf protein concentrate

Control IFW IFN IDW IDN
Non-essential
 Aspartic 2.32 3.13 2.16 1.80 2.16
 Serine 1.34 0.97 0.04 0.59 1.85
 Glutamic 2.10 3.96 4.37 2.07 0.71
 Glysine 1.87 2.27 0.01 1.02 0.01
 Alanine 1.22 2.43 0.33 0.96 0.05
 Proline 1.74 1.36 1.63 0.76 0.01
 Cysteine 0.31 0.16 0.19 0.03 0.05
 Tyrosine 1.28 1.18 1.10 0.69 0.73
Essential
 Methionine 0.43 0.76 0.46 0.29 0.03
 Arginine 1.88 1.87 1.96 0.97 0.04
 Threonine 0.14 1.31 0.26 0.73 2.02
 Histidine 0.84 0.89 0.82 0.41 0.62
 Phenilalanine 2.07 2.37 1.89 1.04 1.30
 Lysine 1.59 2.25 2.19 0.95 0.02
 Valine 1.84 2.80 2.41 1.26 0.28
 Leusine 2.28 3.69 2.88 1.64 1.99
 Isoleusine 1.60 2.38 1.77 1.06 1.28
 Total AA 24.85 33.78 24.47 16.27 13.15
 Total EAA 12.67 18.32 14.64 8.354 7.58

Control=Indigofera leaf meal, IFW=Fresh Indigofera leaves extracted with distilled water, IFN=Fresh Indigofera leaves extracted with 0.1 N NaOH, IDW=Dried Indigofera leaves extracted with distilled water, IDN=Dried Indigofera leaves extracted with 0.1 N NaOH. The data were obtained from the analysis of 5 replicates that were composited, AA=Amino acids, EAA=Essential amino acids