Extended Data Fig. 9. Solvent accessible pore radius.

The pore radius accessible to solvent was estimated using CHAP software ⁶⁴. A. (Left) The surface representation of the accessible pore is shown with the ribbon diagram of Open-Na260. GluA2: light gray. TARPγ-2: dark gray. Pore facing and pore lining residues are in yellow and orange, respectively. The darkness of orange surface corelates with narrower radius of the pore. The value of s is the coordinate along the pore pathway, whose origin is set at the center of mass of the pore forming residues. The relations between the key pore residues and the value of s (in the unit of nm) are shown. (right) Radius vs. s plot. The location of the site-G plus N619 and SF are in blue and green, respectively. The dashed line indicates 1.4Å. The radius of closed pore (Closed-CaNaMg) plotted in B crosses below the 1.4Å radius but the open pores don’t. Radius vs. s plots are shown for Open-Na110 (C), Open-Na610 (D), Open-CaNaMg/N619K (E), Open-CaNaMg (F), Open-Ca10 (G), and Open-Ca150 (H).