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. 2024 Nov 5;24:1354. doi: 10.1186/s12885-024-13108-6

Table 2.

PCR conditions

Steps Temperature Time
Initial Denaturation 95◦C 10 min
Denaturation 95◦C 45 s
Annealing 60◦C 45 s
Extension 72◦C 45 s
Final extension 72◦C 7 min
Storage 4◦C