Skip to main content
. 2024 Sep 17;12(11):e00828-24. doi: 10.1128/spectrum.00828-24

Fig 4.

Timeline of in vivo imaging and antibacterial treatment in mice after infection, survival curves comparing treatments, radiance images depicting infection progression, and radiance intensity for treatment groups over time.

Evaluation of the number of lung-colonizing bacteria of A. baumannii clinical isolates and its application to the assessment of the therapeutic efficacy of antimicrobial agents. Clinical isolates of A. baumannii (NR1127-Luc, a carbapenem-sensitive strain, and NR4001-Luc, a carbapenem-resistant strain) were intratracheally administered to immunodeficient mice at 5 × 107 and 1 × 108 CFU/mouse, respectively. TokeOni was then administered intraperitoneally for in vivo imaging, and saline (i.p.), imipenem/cilastatin (IPM/CS) (i.p.), or levofloxacin (LVFX) (s.c.) was administered for treatment at the indicated time points post-infection. Experiments were performed using five mice per group (A). Kaplan–Meier plot of the NR1127-Luc strain (B) and NR4001-Luc strain (E). Representative images of the NR1127-Luc strain (C) and NR4001-Luc strain (F) acquired using in vivo imaging. The signal obtained from all images of the NR1127-Luc strain (D) and NR4001-Luc strain (G) was plotted for each time point. Red symbols represent the results at the final imaging time points.