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. 2005 Jun 20;33(11):3479–3491. doi: 10.1093/nar/gki658

Figure 6.

Figure 6

CRX transactivates RP1, GUCY2D and ABCA4 in transient transfection assays. (A) Schematic diagram showing the luciferase reporter constructs carrying upstream regions of RP1 (−86 to +33), GUCY2D (−134 to +64), ARR3 (−297 to +16), BBS4 (−151 to +33) and ABCA4 (−130 to +8) in the pGL2-basic vector. The positions of CRX core binding sites (TAAT) are labeled by crosses. (B) Transient transfection assays. GripTite 293 MSR cells were transfected with 0.2 μg of the indicated luciferase reporter construct shown in (A) and increasing amounts (0, 0.2, 1 or 2 μg) of the CRX expression vector pcDNA3.1/HisC-Crx. The fold stimulation was calculated relative to control transfections without pcDNA3.1/HisC-Crx. Error bars show the standard error, n = 3.