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. 2024 Nov 5;14(48):35438–35446. doi: 10.1039/d4ra06573e

Investigated FRET substrates for coronavirus Mprosa.

FRET substrate Structure Length (amino acids)
Blanchard 2-Abz-SVTLQ↓SGY(NO2)R 10
Blanchard-VV 2-Abz-VVTLQ↓SGY(NO2)R 10
PEDV 1 2-Abz-YNSTLQ↓SGY(NO2)R 11
PEDV 2 2-Abz-NSTLQ↓SGY(NO2)R 10
PEDV 3 2-Abz-STLQ↓SGY(NO2)R 9
PEDV 4 2-Abz-STLQ↓AGY(NO2)R 9
(−) FRET (neg. control) 2-Abz-STLAAGY(NO2)R 9
a

Abbreviations: 2-Abz, 2-aminobenzoic acid; Y(NO2), 3-nitrotyrosine.