TABLE 1.
Primers and probes used for Taqman detection of the gB regiona
| Primer or probe | Sequence | Label | Tm (°C) | μM |
|---|---|---|---|---|
| HSV1-F | GCAGTTTACGTACAACCACATACAGC | 59.9 | 0.9 | |
| HSV2-F | TGCAGTTTACGTATAACCACATACAGC | 59.5 | 0.9 | |
| HSV1&2-R | AGCTTGCGGGCCTCGTT | 60.5 | 0.9 | |
| HSV1-probe | CGGCCCAACATATCGTTGACATGGC | 5′FAM, 3′TAMRA | 70.3 | 0.2 |
| HSV2-probe | CGCCCCAGCATGTCGTTCACGT | 5′FAM or JOE, 3′ TAMRA | 70.0 | 0.2 |
a
FAM, 6-carboxyfluorescein; JOE, 6-carboxy-4′, 5′-dichloro-2′, 7′-dimethoxyfluorescein, TAMRA, 6-carboxytetramethyl-rhodamine. Nucleotides that differ from HSV-1 are underlined.