TABLE 7.

Amino acids encoded by codons with nonsynonymous mutations in plasminogen activator gene pauA of S. uberis

Allele	No. of isolates	Sourcea	Codonb
			15	23	40	73	82	115	124	211	240	242
1	15	USA (10), NL (5)	Gly	Val	Pro	Asp	Ala	Arg	Arg	Arg	Pro	Asp
2	3	USA	Gly	Val	Pro	Asn	Ala	Arg	Arg	Arg	Pro	Asp
3	1	USA	Gly	Val	Pro	Asp	Ala	Arg	Arg	Arg	Pro	Asp
4	13	USA (8), NL (5)	Gly	Val	Pro	Asp	Ala	Gln	Gln	Leu	Gln	His
5	1	USA	Ala	Val	Pro	Asn	Ala	Arg	Arg	Leu	Pro	Asp
6	2	USA	Gly	Val	Pro	Asn	Ala	Arg	Arg	Argc	Pro	Asp
7	1	USA	Gly	Val	Pro	Asp	Thr	Arg	Arg	Arg	Pro	Asp
8	1	USA	Gly	Ile	Pro	Asp	Ala	Arg	Arg	Arg	Pro	Asp
9	6	USA (2), NL (4)	Gly	Val	Pro	Asp	Ala	Arg	Arg	Ser	Pro	Asp
10	4	NL	Gly	Val	Pro	Asp	Ala	Arg	Arg	Leu	Pro	Asp
11	1	NL	Gly	Val	His	Asp	Ala	Arg	Arg	Leu	Gln	Asp

^aIsolates originated from bovine milk and were collected from bulk tank milk in the United States (USA; n = 29) and from quarter milk samples in The Netherlands (NL; n = 19). Two additional isolates, one from the United States and one from The Netherlands, did not produce pauA amplicons.

^bAmino acids differing from the majority are bolded.

^cArg is encoded by CGA in allele 6 and by CGC in other alleles with an Arg codon.