FIG. 1.

Fluorescence microscopic analyses of HeLa cells infected with clinical isolates (multiplicity of infection, 5 to 10) containing an incA gene encoding full-length IncA (A) or an incA gene with insertions, leading to premature stop codons (B, C). Immunostaining with anti-MOMP revealed a single inclusion in HeLa cells infected with C. trachomatis isolates (n = 86), containing full-length IncA, irrespective of its ST. In contrast, multiple small inclusions were observed in HeLa cells infected with strain 190-163-02 (B) or strain 190-059-03 (C) carrying an incA with a premature stop codon. Bar, 10 μm.