TABLE 1.

Primers used for the MLST of the 85 Salmonella enterica serovar Typhimurium clinical isolates

Gene	Direction	Primer sequence	Approx amplicon size (bp)a	Reference or source
spaM	Forward:	5′-CGCTGTACGGTATTTCATT-3′	394	12
	Reverse:	5′-CTGACTCGGCCTCTTCCTG-3′		This studyb
manB	Forward:	5′-CCGGCACCGAAGAGA-3′	893	14
	Reverse:	5′-CGCCGCCATCCGGTC-3′		14
pduF	Forward:	5′-CTCAAAGTCGCYGGYGC-3′	518	14
	Reverse:	5′-GGGTTCATTGCAAAACC-3′		14
glnA	Forward:	5′-CCGCGACCTTTATGCCAAAACCG-3′	474	14
	Reverse:	5′-CCTGTGGGATCTCTTTCGCT-3′		14

^aThe length of the sequenced DNA in both strands was slightly shorter.

^bThis primer was designed using the DNA sequence of GenBank accession no. SEU43315.