TABLE 2.

Oligonucleotide primers for LAMP

Primer	Type	Sequencea	Amplicon size (bp)	Target	Source (Strain)
Porphyromonas gingivalis
Pg-F3	F3	5′-GGC AGT AAT CGG CGC ATT-3′	225	pepO	381
Pg-B3	B3	5′-TCG TGC AGG ATG TCG AAT G-3′
Pg-FIP	FIP	5′-ACT GAG GTC GAT GGC CGG TAG CTG CAA TGG CAA TAA GGG T-3′
Pg-BIP	BIP	5′-CCG CAG GAC GAC TTT TAT CGC TAG CCG TAG CGA CTA TAA GCA-3′
Pg-LF	LF	5′-GCT TCC TGT CAG TAT CGT TAG TCT G-3′
Pg-LB	LB	5′-ACT GCA ACG GCA ATT GGA TG-3′
Tannerella forsythia
Tf-F3	F3	5′-GCA ACC AAG ATT GCC AGA GA-3′	219	cct	ATCC 43037
Tf-B3	B3	5′-AAC AGC GAC TGC AAC GAA-3′
Tf-FIP	FIP	5′-GGC ACC ACA CAG GAA CGA GTT AGG GAA TTG CCA AGG ATG TCA-3′
Tf-BIP	BIP	5′-GGG TAA GCC AAC GGT AGA GAC CGG AAT CTG CAT TCA CAC-3′
Tf-LF	LF	5′-ACG TAGTTG CCG GTG TCA-3′
Tf-LB	LB	5′-TCA GTT CCG CCA AGT CAA TG-3′
Treponema denticola
Td-F3	F3	5′-AAA GGC TTT GGG CGA CAG-3′	240	opdB	ATCC 35405
Td-B3	B3	5′-TCC CGT CCT CAT ACC ACT TT-3′
Td-FIP	FIP	5′-GGT GAG GAC CCG TCC TTT ACC ACG GAG TGA AGG TGC CTA TG-3′
Td-BIP	BIP	5′-GCT CCG ATG CGT TTT TCA GTC CGC CCC TGA TTT GAG CAA CA-3′
Td-LF	LF	5′-AAC CTT TTT TGT AAA CGG CAG C-3′
Td-LB	LB	5′-GAG TGT TTA CAG CCT TGT AGA GAG G-3′

^aAccession numbers: AB010440 for Porphyromonas gingivalis pepO gene; AY368075 for Tannerella forsythia cct gene; and AF355459 for Treponema denticola opdB gene.