TABLE 1.

Primer and probe sets for real-time RT-PCR^a

Target gene	Primer or probe	Sequence (5′→3′)	Tm (°C)	Location
N gene	FP	AGGAACTGGCCCAGAAGCTT	59	28461-28480
	RP	AACCCATACGATGCCTTCTTTG	59	28500-28521
	Probe	ACTTCCCTACGGCGCTA	69	28482-28498
ORF 8	FP	ACTACTAAAAGAACCTTGCCCATCAG	59	27359-27384
	RP	TATTGTCAGCAAGAGGGTGAAATG	59	27406-27429
	Probe	TGAATTGCCCTCGTATGTT	70	27386-27404
ORF 3	FP	TCTCCTGCAAGTACTGTTCATGCT	59	25337-25360
	RP	GCCATCCGAAAGGGAGTGA	60	25385-25403
	Probe	CAACGATACCGCTACAAG	69	25365-25382

^aAbbreviations: FP, forward primer; RP, reverse primer; T_m, melting temperature. The target gene name and location are based on the SARS-CoV Tor2 strain (GenBank accession number AY274119).