TABLE 3.
Results of the real-time RT-PCR assay with specimens from patients and masked palm civetsa
| Specimen no. | Real-time RT-PCR CT valueb
|
Log10 virus concn (no. of copies/ml)c
|
||||
|---|---|---|---|---|---|---|
| N gene | ORF 3 | ORF 8 | N gene | ORF 3 | ORF 8 | |
| H2003001 | 30.06 | 37.49 | 38.34 | 5.06 | 3.41 | 3.46 |
| H2003002 | 28.46 | 35.54 | 36.76 | 5.54 | 4.01 | 3.90 |
| H2003003 | 13.3 | 18.42 | 18.22 | 10.11 | 9.31 | 9.20 |
| H2003004 | 10.95 | 15.67 | 15.63 | 10.81 | 10.17 | 9.93 |
| H2003005 | 33.99 | 36.25 | 37.74 | 3.87 | 3.79 | 3.63 |
| H2003501 | ND | ND | ND | |||
| H2003502 | ND | ND | ND | |||
| H2003503 | ND | ND | ND | |||
| H2003504 | ND | ND | ND | |||
| MPC200404081 | 29.13 | ND | ND | 5.81 | ||
| MPC200404084 | ND | ND | ND | 2.54 | ||
| MPC200404085 | 22.57 | 41.28 | 40.34 | 7.99 | 2.7 | 3.36 |
| MPC200404088 | 28.06 | ND | ND | 6.12 | ||
| MPC200404252 | ND | 27.72 | 42.66 | 6.9 | 2.70 | |
| MPC200404256 | ND | 41.89 | 43.29 | 2.51 | 2.52 | |
| MPC200404258 | ND | N | 42.16 | 2.84 | ||
a
Abbreviations: H, human; MPC, masked palm civet; ND, not detected.
b
The mean of triplicate values.
c
Calculated from the corresponding CT values by using standard curves generated from RNA transcripts.