Figure 2.

The inhibitory effect of different concentrations of shikonin on migratory and invasive abilities of EC9706 cells. (A) The size of the scratches measured by inverted fluorescence microscopy at 0 and 48 h in the three different groups of EC9706 cells. The images were captured at 40×. Histograms provide quantitative data on wound healing. (B) The Transwell assay detected the migration and invasion of EC9706 cells, which penetrate the polycarbonate membrane in the lower chamber that are stained purple. Compared with the normal control (NC) group, ⁣^∗∗P < 0.01 and ⁣^∗∗∗P < 0.001.