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. 2024 Nov 5;30(11):e70109. doi: 10.1111/cns.70109

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© 2024 The Author(s). CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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MPO immunodepletion decreased ROS levels in SH‐SY5Y cells. (A) One‐way ANOVA revealed a significant effect of immunodepletion ([F (2, 9) = 24.7], p = 0.0002) on MPO activity in SH‐SY5Y cells. Specifically, Tukey's test revealed a significant decrease in MPO activity in XDP(−) treated cells compared with both XDP (p = 0.011) and XDP(+) treated cells (p = 0.0003). (B) One‐way ANOVA demonstrated a significant effect of treatment ([F (3, 12) = 9.763], p = 0.0015) on MPO activity in SH‐SY5Y cells. Tukey's test demonstrated a significant increase in MPO activity in XDP‐treated cells compared with vehicle‐treated cells (p = 0.0460) as well as a significant decrease in MPO activity in XDP(−) cells compared with both XDP (p = 0.0020) and XDP(+) treated cells (p = 0.0066). (C) Two‐way ANOVA revealed a significant effect of immunodepletion ([F (4, 48) = 32.85], p < 0.0001) and time ([F (12, 48) = 16.96], p < 0.0001) on ROS levels in SH‐SY5Y cells. As expected, there was an increase in ROS in antimycin A treated cells compared with vehicle treated cells (Tukey's test, p < 0.0001). Additionally, Tukey's test demonstrated a significant increase in ROS in XDP (p < 0.0001) and XDP(+) treated cells (p < 0.0001) compared with vehicle treated cells. Importantly, Tukey's test also revealed a significant decrease in ROS in XDP(−) treated cells compared with both XDP (p < 0.0001) and XDP(+) treated cells (p < 0.0001). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.