Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| AlexaFlour647 anti-biotin IgG | Jackson ImmunoResearch Laboratories | Cat# 200-602-211, RRID:AB_2339046) |
| Anti-CD20 | InvivoGen | Cat# hcd20-mab10, RRID:AB_11124933 |
| Mouse Anti-CD16/32 | Cell signaling | Cat# 88280 |
| Anti-Rat IgG biotin conjugate | Invitrogen | Cat# 13-4813-85 |
| Anti-CD45 | Cell Signaling | Cat# 70257T |
| Anti-Rabbit 488 | Cell Signaling | Cat# 4412S |
| Anti-CD44 Superbright 600 | Invitrogen | Cat# 63-044-82 |
| Anti-CD64 PerCP-eFuor710 | Invitrogen | Cat# 46-0641-80 |
| Anti-CD16-2 FITC | Invitrogen | Cat# MA-28253 |
| Anti-CD32b APC | Invitrogen | Cat# 17-0321-80 |
| Anti-CD16/32 PE | Biolegend | Cat# 101307 |
| Anti-SIRPa APC/Cy7 | Biolegend | Cat# 144018 |
| Superbright 600 isotype control | Invitrogen | Cat# 634031-82 |
| PerCP-eFuor710 isotype control | Invitrogen | Cat# 46-4714-80 |
| FITC isotype control | Biolegend | Cat# 402307 |
| APC isotype control | Invitrogen | Cat# 17-4724-81 |
| PE isotype control | Invitrogen | Cat# 12-4321-80 |
| APC/Cy7 isotype control | Biolegend | Cat# 400422 |
| Chemicals, peptides, and recombinant proteins | ||
| POPC | Avanti | Cat# 850457 |
| Biotinyl cap PE | Avanti | Cat# 870273 |
| PEG5000-PE | Avanti | Cat# 880230 |
| Atto390-DOPE | ATTO-TEC GmbH | Cat# AD 390-161 |
| Atto647-DOPE | ATTO-TEC GmbH | Cat# AD 647-161 |
| Ni2+-DGS-NTA | Avanti | Cat# 790404 |
| DOPS | Avanti | Cat# 840035 |
| CellTrace Far Red | ThermoFisher | Cat# C34572 |
| CellTrace CSFE | ThermoFisher | Cat# C34570 |
| Qdot 655 | ThermoFisher | Cat# Q10123MP |
| Casein | Sigma | Cat# C5890 |
| PD0325901 - ERK inhibitor | Sigma | Cat# PZ0162 |
| Actinomycin D | Cell signaling | Cat# 15021s |
| Cycloheximide | Cell signaling | Cat# 2112s |
| dNTPs | New England Biolabs | Cat# N0447l |
| RNase OUT | ThermoFisher | Cat# 10777019 |
| Superscript II Reverse Transcriptase | Invitrogen | Cat# 1864014 |
| E. coli DNA Polymerase I | Invitrogen | Cat# 18010025 |
| RNase H | ThermoFisher | Cat# EN0202 |
| DNA magnetic beads | AMPure | Cat# A63882 |
| MEGAscript T7 | Invitrogen | Cat# A57622 |
| ExoSAP IT PCR reagent | Invitrogen | Cat# 78200.200.UL |
| LPS | Sigma | Cat# L4516 |
| IFN-y | Sino Biological | Cat# 50709-MNAH |
| IL-4 | Sino Biological | Cat# 51084-MNAE |
| Critical commercial assays | ||
| Pierce Fab Preparation Kit | ThermoFisher | Cat# 44985 |
| PureLink RNA mini kit | Invitrogen | Cat# 12183018A |
| Experimental models: Cell lines | ||
| Human: HEK239T | ATCC | Cat# ATCC CRL-3216 |
| Mouse: RAW264.7 | ATCC | Cat# ATCC TIB-71; RRID:CVCL_0493 |
| Mouse: L929 | ATCC | Cat# ATCC CLL1; RRID:CVCL_0462 |
| Sf9 | ThermoFisher | Cat# 11496015 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6 | The Jackson Laboratory | Cat# 000664 |
| Recombinant DNA | ||
| pHR-optoFcR | This paper | In pHR vector. Myristolization sequence: MGSSKSKPKDPSQ R; cytoplasmic domain (aa 45–86) of the Fc γ-chain UniProtKB - P20491 (FCERG_MOUSE); linker: STSG; fluorophore: mScarlet; linker: SDPGSGS; Cry2-olig (aa 1-498) of CRY2_ARATH UnitprotKB – Q96524 with E490G mutation followed by: ARDPP (as described in Taslimi et al, 2014) |
| pHR-Syk NeonGreen NK83 | Kern et al.21 | Addgene plasmid # 176610 ; http://n2t.net/addgene:176610 ; RRID:Addgene_176610 |
| pHR mCherry-caax | Morrissey et al.47 | mCherry fused to membrane targeting sequence from KRAB (amino acids LEKMSKDGKKKKK KSKTKCVIM) |
| pMD2.G | pMD2.G was a gift from Didier Trono, Swiss Federal Institute of Technology Lausanne | Addgene plasmid # 12259 ; http://n2t.net/addaene:12259 : RRID:Addgene_12259 |
| pCMV-dR8.2 | pCMV-dR8.2 dvpr was a gift from Bob Weinberg, Whitehead Institute for Biomedical Research | Addgene plasmid # 8455 ; http://n2t.net/addaene:8455 ; RRID:Addgene_8455 |
| ICAM-tagBFP-His10 | O’Donoghue et al.55 | N/A |
| Software and algorithms | ||
| ImageJ -Fiji | NIH | RRID:SCR_002285 https://fiji.sc/ |
| Affinity Designer | Serif | RRID:SCR_016952 |
| Prism | Graphpad | RRID:SCR_002798 |
| TrackMate | Ershov et al.64; Tinevez et al.65 | N/A |
| Moment Scaling Spectrum analysis | This paper | https://github.com/MZW-Lab/Trajectory_Analysis_optoFcR/tree/main; https://doi.org/10.5281/zenodo.12701581 |
| Blind-Analysis-Tools-1.0 | Github | https://github.com/ahtsaJ/Blind-Analysis-Tools |
| JaCoP | Bolte et al.54 | N/A |
| FlowJo 10 | FlowJo | RRID:SCR_008520 |
| RNAseq analysis | Atkins et al.59 | N/A |
| Other | ||
| 5 um silica beads | Bangs Labs | Cat# SS05003 |
| MatriPlate | Brooks | Cat# MGB09-1-2-LG-L |
| LITOS stimulation plate | Hohener et al.53 | N/A |
| Alexa Fluor 647 MESF calibration beads | Bangs Labs | Cat# 647 |
| RNAseq data | This paper | https://doi.org/10.5061/dryad.hx3ffbgp1 |