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. Author manuscript; available in PMC: 2024 Nov 6.
Published in final edited form as: Dev Cell. 2024 Jun 7;59(21):2818–2833.e7. doi: 10.1016/j.devcel.2024.05.019

Figure 5: Dermal macrophage gene expression analysis and role for Axl signaling in effects of TRPV1 neuronal activation on fibroblasts.

Figure 5:

(A) UMAP projection demonstrating the clustering of sorted dermal CD45+ cells based of scRNAseq data.

(B) UMAP projection demonstrating the expression of TRGV4, TRBC1, CD207, H2-Ab2, Itgax, Itgam, Xcr1, Fcgr1 and Mertk.

(C) Visualization of CellChat data demonstrating potential interactions and their probability between cluster #5 of dermal macrophages and cluster #3 of dermal fibroblasts.

(D) Heat map visualization of gene expression for ligands identified by CellChat to have potential interactions with fibroblasts (x-axis) across all clusters (y-axis). Rectangle separates macrophage defining markers (Mertk and Fcgr1). Color encodes average gene expression.

(E) UMAP projection of sorted fibroblasts demonstrating the expression of Axl.

(F) Percentage of dermal CD26+CD9+ fibroblasts from TRPV1 activated and control mice pretreated with R428 or vehicle (**p=0.004,****p<0.0001).