TABLE 5.
Molecular siblings of medically important Aspergillus species investigated in this study
| Query speciesa | Molecular siblingb | Maximum sequence identity (%) per target (minimum nucleotide position difference)c
|
||
|---|---|---|---|---|
| D1-D2 | ITS1 | ITS2 | ||
| A. candidus | A. campestris | 100 (0) | NAd | NA |
| A. (Eurotium) chevalieri | Various Eurotium speciese | 100 (0) | 100 (0) | 100 (0) |
| A. (Fennellia) flavipes | None | |||
| A. flavus | A. oryzae | 100 (0) | 100 (0) | 100 (0) |
| A. parasiticus | 100 (0) | 97.8 (4) | 97.7 (4) | |
| A. sojae | 100 (0) | 97.8 (4) | 97.7 (4) | |
| A. tamarii | 99.8 (1) | 95.6 (8) | 97.0 (5) | |
| A. subolivaceus | 100 (0) | NA | NA | |
| A. terricola | 100 (0) | NA | NA | |
| A. flavofurcatus | 99.8 (1) | NA | NA | |
| A. fumigatus | Various Neosartorya speciese | 99.8 (1) | 98.9 (2) | 97.0 (5) |
| A. granulosus | A. ustus | 99.8 (1) | 94.3 (9) | 98.3 (3) |
| A. puniceus | 99.6 (2) | 84.3 (27) | 98.3 (3) | |
| A. pseudodeflectus | 99.8 (1) | NA | NA | |
| A. (Emericella) nidulans | Various Emericella speciese | 100 (0) | 100 (0) | 100 (0) |
| A. niger | A. awamori | NA | 100 (0) | 100 (0) |
| A. foetidus | NA | 100 (0) | 100 (0) | |
| A. phoenicis | 100 (0) | 98.9 (2) | 99.4 (1) | |
| A. restrictus | A. caesiellus | 99.8 (1) | 99.4 (1) | 96.5 (6) |
| A. conicus | 100 (0) | NA | NA | |
| A. sydowii | A. versicolor | 99.1 (5) | 98.1 (3) | 98.2 (3) |
| A. terreus | Fennellia nivea | 100 (0) | 92.6 (14) | 91.5 (15) |
| A. carneus | 100 (0) | NA | NA | |
| A. allahabadii | 99.8 (1) | NA | NA | |
| A. ustus | A. granulosus | 99.6 (2) | 92.5 (12) | 98.3 (3) |
| A. puniceus | 100 (0) | 89.5 (18) | 100 (0) | |
| A. pseudodeflectus | 99.8 (1) | NA | NA | |
| A. versicolor | A. sydowii | 99.3 (4) | 98.1 (3) | 98.8 (2) |
See Table 1 for the origin of strains used and for the corresponding GenBank sequence accession numbers; sequence data for only the type strains or reference strains with identical ribosomal sequences compared to corresponding type strains are shown for ease of presentation because despite some nucleotide variations among sequevars of a given species, molecular siblings captured from GenBank were the same for all sequevars within a given species (see Results section).
Organism(s) assigned to a different species than query sequence although exhibiting identical or very similar (>99% identity) sequences in at least one ribosomal region investigated in this study. The listing is limited to Aspergillus species currently sanctioned by the International Commission of Penicillium and Aspergillus (37). Note that the query sequences of A. chevalieri, A. flavus, A. nidulans, A. niger, A. sydowii, and A. versicolor also revealed “false” molecular siblings in GenBank, i.e., GenBank sequences with ambiguous classification as detailed in the Results section of this study.
Comparison between query sequences (this study) and reference sequences of molecular siblings obtained from GenBank (15, 35) and the present investigation (A. granulosus ITS sequences) using BLAST and GCG algorithms.
NA, no corresponding reference data available for this molecular sibling in this target region at the time this study was conducted.
Query sequences matched multiple species of this genera in the D1-D2 and ITS regions although ITS reference data for many species of this taxonomic group were not available in GenBank at the time this study was conducted.