TABLE 2.
Oligos for analyzing influenza B gene fragments
| Gene | Oligos for PCR or sequencing | Nucleotide positions | GenBank accession no. of reference sequence |
|---|---|---|---|
| NAa | 5′-GCTACCTTCAACTATACAAACG | 56-1549 | AY139074 |
| 5′-CAAGAGCATTTTTCAGAAAC | |||
| NP | 5′-GAAGTAGGTGGAGACGGAGGG | 1412-1802 | M20173 |
| 5′-GTAAACACCCACATTCCAAACG | |||
| NS | 5′-GAGCAGCTGAAACTGCGGTG | 810-1066 | M20224 |
| 5′-CACAAGCACTGCCTGCTGTAC | |||
| M | 5′-ATGTCGCTGTTTGGAGACAC | 25-320 | M20175 |
| 5′-GCTAGAATCAGGCCTTTCTT | |||
| PA | 5′-CCTGAATTACAACCAGCAATGC | 111-463 | M20171 |
| 5′-AGTTCCATGCTATTTCCCAG | |||
| PB1 | 5′-ACCGCTGGAATACAAATCAG | 748-1011 | M20169 |
| 5′-TGGGCTGTCTCTGGTTATTC | |||
| PB2 | 5′-CTGTTAAGGGACAATGAAGC | 57-301 | M20163 |
| 5′-CACATTTGGCCTTTGGTTCC |
a
Six additional oligo primers were also used for sequencing the NA gene fragment: 5′-ATTGTAGTATCC CCCTGGTT, 5′-ACTCCGATATATGTCCATTC, 5′-TTCCACATGGCAGCTTGGAG, 5′-TCTTAAGATTCGAGAGGGCC, 5′-TTATCTCTACAGGCACATTC, and 5′-GTACCACCTTCCAATCTTGG.