FIGURE 4.

Growth of H. pylori is inhibited by P. volubilis water infusion, EA extract and astragalin. Broth microdilution assays were used to expose a clarithromycin-resistant clinical isolate of H. pylori (SSR366) to increasing concentrations of P. volubilis water infusion (A), ethyl acetate extract (B) and astragalin (C). Data points represent mean and standard deviation (error bars) of triplicate values. The curve was generated using non-linear regression. The graphs were then analysed using the Gompertz fit model to determine the MIC values (Table 3).